Table 4.
Protein identification of each spot
| Spot number | Protein | Score | Coverage (%) | Mass (kDa) | pI |
|---|---|---|---|---|---|
| 85 | Inter-alpha-trypsin inhibitor heavy chain H4 | 2,904 | 23 | 103,552 | 6.51 |
| Inter-alpha-trypsin inhibitor heavy chain H1 | 518 | 12 | 101,844 | 6.31 | |
| 421 | Kininogen-1 | 1,313 | 18 | 73,041 | 6.34 |
| Angiotensinogen | 464 | 15 | 53,439 | 5.87 | |
| 16 | Haptoglobin | 524 | 26 | 39,521 | 6.13 |
| Haptoglobin | 376 | 20 | 45,889 | 6.42 | |
| Complement C4 | 248 | 3 | 194,367 | 6.65 | |
| 113 | Haptoglobin | 925 | 36 | 45,889 | 6.13 |
| Haptoglobin | 767 | 24 | 39,521 | 6.42 | |
| apoA | 279 | 35 | 45,398 | 5.28 | |
| 10 | Haptoglobin | 507 | 26 | 45,889 | 6.13 |
| Complement C4 | 478 | 2 | 194,367 | 6.65 | |
| Haptoglobin | 381 | 22 | 39,521 | 6.42 | |
| 4 | Haptoglobin | 391 | 26 | 45,889 | 6.13 |
| Haptoglobin-related protein | 284 | 22 | 39,521 | 6.42 | |
| 624 | Complement C3 (fragment?) | 697 | 12 | 188,686 | 6.02 |
| Gelsolin | 413 | 10 | 86,096 | 5.90 | |
| Serum albumin | 222 | 17 | 71,363 | 5.92 | |
| Ig mu chain C region | 216 | 12 | 49,991 | 6.35 |
Each spot was excised from the 2-D electrophoresis gel and the protein reduced to peptides by trypsin. The mass of each peptide was determine by LCMS and matched to known proteins in the Mascot database using the guidelines recommended by Matrix Science (Boston, MA, USA). The molecular weight of several proteins is greater than the position of that spot on the gel. These may be a fragment of the higher molecular weight protein