Fig. 7.
Effect of diet on H2S-induced contractions. CR causes an increase in contraction magnitude in response to H2S in the first-phase contraction (a, n = 18 AL, n = 23 CR), second-phase relaxation (b, n = 18 AL, n = 23 CR) and third-phase contraction (c, n = 18 AL, n = 23 CR). The y-axis represents the H2S-induced tension of aortic rings (grams per milligram) pooled from all ages. The x-axis represents H2S concentration. Columns show diet for ad libitum (AL, white) or caloric restricted (CR, grey) animals at each concentration tested. Data are presented as box plots showing the median as a horizontal line, the box covering the 25th and 50th percentiles, and error bars defining the 10th and 90th percentiles. Data were analyzed by one-tailed t test, pooled t test, or Wilcoxon's test. Medians that are significantly different are indicated by asterisks