Fig. 2.

Changes in PR gene RNA levels in response to whitefly feeding. A Tomato plants were infested with Besimia tabaci biotype B (Bt) or Trialeurodes vaporariorum (Tv) or served as non-infested controls (C). Control leaves were encased and nylon bags but insects were not added as described in Fig. 1A. Total RNA blots were hybridized with ³²P-labeled basic β-1,3-glucanase (GluB), basic chitinase (Chi9), PR-1 (P6), PR-4, acidic chitinase (Chi3), or acidic β -1,3-glucanase (GluAC) cDNA probes. Experiments were repeated once and each lane is pooled leaf material from three plants. Film exposure times were: 3 d for Chi9, GluB, and PR-4; 2 d for PR-1, 4 days for Chi3; 6 d for GluA, B, C Plants were infested as in Fig. 1B. Infested and apical, non-infested leaves were harvested 0, 3, 5, 7, and 9 d after B. tabaci biotype B (Bt) or T. vaporariorum (Tv) infestation. Controls were leaves from bagged but non-infested (Control B) or untreated (Control U) plants. RNA blots were hybridized to each ³²P-labeled PR gene probe in Panel A; only data from the GluB (Panel B) or PR-1 (Panel C) probes are shown in Panel B. Stained gels visualizing rRNAs are shown as a loading control. Film exposures time were 2 d