Fig. 3.

RNA blot analyses of wound- and defense-response RNAs in tomato plants treated with defense signals. Excised tomato seedlings were treated with 10 μM MeJA, 29 ppm ethylene (Eth), 100 μM ABA, 1.0 pmol systemin (Sys), or 0.1, 0.25, or 0.5 mM SA. For each treatment, the corresponding control is shown (C). Total RNA blots were hybridized to ³²P-labeled PAL, LapA, Chi9, Chi3, and GluAC probes. Transcript sizes are shown in kb and were determined using a RNA marker run in a parallel lane. Stained gels visualizing rRNAs are shown as a loading control. The stained gel for the PAL5 blot is not shown. Exposure times were 21–23 hr