Skip to main content
. Author manuscript; available in PMC: 2010 Nov 13.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7381–7388. doi: 10.4049/jimmunol.0804322

Figure 4. Notch activation is necessary for Dll4 enhanced IL17 production in CD4+ T cells.

Figure 4

A) CD4+ T cells were MACS purified from the spleen of Balb/c mice and were skewed under Th17 conditions in the presence or absence of Dll4 or GSI or control (DMSO) for 48 hours. The supernatants were analyzed for IL17 production. The data represents the mean ± the SEM of triplicate determinants and are representative of four independent experiments. * p = 0.004 and ** p = 0.002. B) CD4+ T cells were MACS purified from the spleen Balb/c mice and were skewed under Th17 conditions in the presence or absence of Dll4 for 4 hours or 20 hours. The whole cell lysate was analyzed by Western blot for the notch intracellular domain (N-ICD) and β-actin was used as loading control. The experiment was repeated twice with similar results. C) Using the same conditions as above Dll4 enhances not only IL-17 but also the mRNA expression of IL-22 and the Notch pathway target gene hes5. Data represents the mean ± SE from 3 separate experiments.