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. Author manuscript; available in PMC: 2010 Nov 13.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7381–7388. doi: 10.4049/jimmunol.0804322

Figure 5. Differential regulation of transcription factors rorγ-t and gata3 by Dll4.

Figure 5

A) CD4+ T cells were stimulated under Th17 conditions in the presence or absence of rDll4 for varying time. Total RNA was extracted, reverse transcribed and cDNA was amplified using specific primers for rorγ-t or gata-3. The data is expressed as the mean± the SEM. * p = 0.031. B) CD4+ T cells were stimulated under Th17 conditions in the presence or absence of IL6 or TGFβ or rDll4. The whole cell lysate was the analyzed by western blot for phosphorylation of STAT-3. β-actin was used as a loading control.