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. Author manuscript; available in PMC: 2010 Nov 13.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7381–7388. doi: 10.4049/jimmunol.0804322

Figure 7. RBPj-κ (CSL) directly interacts with IL17 promoter on specific binding sites.

Figure 7

CD4+ T cells were activated under Th17 skewing conditions in the presence of rDll4 or in the presence or absence of GSI for 24 hours. The cells were the fixed for ChIP. IP was carried out using anti CSL antibody. The three CSL binding sites on IL17 promoter (p1, p2 and p3) were amplified and analyzed by quantitative RT-PCR. The data represents the normalized percentage amounts relative to the input DNA before IP. The experiment is one representative of three independent experiments. The data is expressed as the mean ± the SE and is representative of four independent experiments.