Figure 1.

Strategy for identification of IR-induced post-translational modifications of H2AX. (A) Outline of protocol for identifying IR-induced post-translational modifications of H2AX. IP: immunoprecipitation; HA: influenza hemagglutinin peptide; MS: mass spectroscopy. (B) Coomassie blue staining of immunoprecipitates after SDS-PAGE analysis. Several histone species are shown. FT: flow-through. uH2A: monoubiquitinated histone H2A. “*” indicates the HA-H2AX bands, which were cut from the gel and purified for analysis by MS.