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Effect of H2AX mutations on I-SceI-induced NHEJ. Percentage of I-SceI-induced GFP+ cells from H2AX+/+ and H2AX−/− NHEJ reporter mouse ES cells (A) or H2AX−/− HR reporter mouse ES cells transiently transfected with mammalian expression vectors encoding H2AX mutants as indicated in (B). Bars represent mean of triplicates. Error bars indicate s.e.m.
