Figure 5.

ACA, O₂, and KCN responses of starved yeast cells. A representative trace of NAD(P)H fluorescence from starved yeast cells treated with ACA, O₂, and KCN is shown. The labels indicate the depletion of O₂ in suspension (a) (see text); addition of 30 μl 0.005 M ACA (b); addition of 15 μl 0.05 M ACA (c); bubbling O₂ through suspension for 10 s (d); bubbling O₂ through suspension for 20 s (e); addition of 100 μl 0.1 M KCN (f); bubbling O₂ through suspension for 10 s (g); and addition of 15 μl 0.05 M ACA (h). Note that huge changes in fluorescence are possible without addition of glucose.