Figure 2.

(a) Temperature ramp of native hNS followed through the far-UV CD signal at 216 nm. In the first segment hNS was heated from 20°C to 70°C (upper trace, green online) and then cooled down to 20°C (middle, purple online). In the third segment hNS was heated to 95°C (middle, blue online) and again cooled down to 20°C (lower trace, red online). The two transitions are highlighted and labeled T1 and T2, respectively. (b) The CD spectra recorded after each step of the temperature ramp are shown at 20°C (upper spectrum, blue online) after being heated at 70°C (middle, red online) and 95°C (lower, green online). (Inset) The same samples analyzed by native PAGE.