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. 2010 Nov 17;99(10):3402–3411. doi: 10.1016/j.bpj.2010.09.021

Figure 3.

Figure 3

(a) Chemical unfolding of native hNS (circles), latent-45 (squares), and latent-85 (triangles), followed through far-UV CD at 216 nm by increasing concentrations of GdHCl (for a more thorough unfolding curve of native hNS and partial unfolding curves for latent forms followed at 222 nm, see Fig. S2). (b) Thermal unfolding (monitored through far-UV CD at 216 nm) of native hNS with 1 M GdHCl, latent-45 with 2 M GdHCl, latent-85 with 3 M GdHCl, polymer-45 with 2 M GdHCl, and polymer-85 with 3 M GdHCl.