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(a) Temperature ramps of hNS latent-45, latent-85, polymer-45, and polymer-85 in the 20–95°C temperature range, monitored through far-UV CD at 216 nm. (b) Far-UV CD spectra of native hNS, latent-45, polymer-45, latent-85, and polymer-85 are superimposed (online: native hNS is blue, latent-45 red, polymer-45 green, latent-85 purple, and polymer-85 cyan). (Inset) Native PAGE of native hNS (N), latent-45 (L45), and latent-85 (L85).
