(a) TEM pictures of polymer-45 and polymer-85. (b) FTIR spectra of monomeric native hNS at 25°C (panel I), hNS polymer-45 (panel II), and hNS polymer-85 (panel III). The amide I band areas (1600–1700 cm−1) are normalized to 100 (open circles). Solid lines are the best fit to the data, obtained as the sum of different spectral components (colored curves online). (Panel IV) Percentage of each amide I band spectral component (color-coded online) as for the spectra deconvolution in panels I–III above. Native hNS, open bars; polymer-45, dotted bars; polymer-85, solid bars.