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. 2010 Nov 17;99(10):3274–3281. doi: 10.1016/j.bpj.2010.09.033

Figure 5.

Figure 5

TM normalized thickness versus PEG MW in isosmotic solutions for wild-type (gray) and TectaY1870C/+ (black) mice. For each MW of PEG, the concentration was adjusted to exert a nominal osmotic pressure of 250 Pa. Thickness was normalized to that measured using 511 kDa PEG. Circles denote the median thickness, vertical lines show the interquartile range. The values for wild-type TMs have been offset horizontally for clarity. Thickness for wild-type TMs levels off for PEG with MW ≥ 200 kDa. For TectaY1870C/+ TMs, thickness continues to decrease with increasing MW.