Figure 2.

Full-length myosin VI molecules. The four panels a–d show molecules under different conditions: (a) apo, (b) ADP, (c) ATP and (d) ATP-γS (all nucleotides 0.5 mM). The upper part of each panel shows the field view with individual myosin VI molecules and residual actin filaments that are used in the myosin purification. Note the straightened shapes of the myosin VI in the presence of nucleotides; also, the heavy decoration of the actin filaments by the myosin VI without nucleotide or in ADP, and the bareness of the actin filaments in ATP and ATP-γS. Arrows in panel d indicate possible detachment of calmodulin lobes.