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Protein expression of AMPK (A), pAMPK (B), p53 (C), and Klotho (D) in heart tissue from young or old C57BL/6 and LID mice. (Inset) Representative gel blots of AMPK, pAMPK, p53, Klotho, and GAPDH (loading control) using specific antibodies against AMPK, pAMPK, p53, Klotho, and GAPDH. Mean ± SEM, n = 4–7 per group, (*) p < 0.05 versus the C57BL/6 young group, (#) p < 0.05 versus the C57BL/6 old group.
