Table 1.
Diversity of rstR Gene of the CTX Prophage and tcpA Gene of the TCP Pathogenicity Island Carried by Environmental and Clinical V. cholerae Strains in Bangladesh
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Type of rstR geneb |
Type of tcpA geneb |
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V. cholerae strain | Number of strainsa | Source | Presence of CTX | rstRClass | rstRET | rstRCal | rstREnv | tcpAClass | tcpAET | ||
Non-O1, non O139 | 3 | Water | + | − | − | − | + | + | − | ||
4 | Water | + | + | − | − | + | + | − | |||
3 | Water | + | − | − | + | + | + | − | |||
10 | Water | − | − | − | − | − | + | − | |||
O139 | 9 | Water | + | − | + | + | − | − | + | ||
O139 | 8 | Patient | + | − | + | + | − | − | + | ||
O1 El Tor | 26 | Water | + | − | + | − | − | − | + | ||
O1 El Tor | 3 | Water | − | − | − | − | − | − | + | ||
O1 El Tor | 17 | Patient | + | − | + | − | − | − | + |
These strains were identified to be positive for CTX or TCP by screening a total of 324 strains isolated from surface water or cholera patients.
Type of rstR and tcpA genes was determined by specific DNA probes or PCR assays.