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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Eur J Pain. 2010 Aug 2;15(2):179–185. doi: 10.1016/j.ejpain.2010.07.001

Table 1.

Primary antibody Dilution Specificity Secondary antibody Dilution
Goat anti-NR1 (Santa Cruz; #sc-1467) 1:100 Polyclonal antibody raised against carboxyl terminus, amino acids 918-938: RRAIEREEGQLQLCSRHREH [Marvizon et. al. (Marvizon et al., 2002)] WB: HRP-conjugated donkey anti-sheep (Jackson Immunoresearch)
IHC: Donkey anti-goat 488 (Molecular Probes)WB:		 1:3000
1:200
Rabbit anti-NR2B (Millipore; #06-600) 1:100 Polyclonal antibody raised againstcarboxyl terminus, amino acids 1463-1482: FNGSSNGHVYEKLSSIESDV [Millipore] WB: HRP-conjugated donkey anti-rabbit (Amersham)
IHC: Donkey anti-rabbit 594 (Molecular Probes)		 1:2000
1:200
Goat anti-GS (Millipore; MAB302) 1:2000 Monoclonal antibody raised against glutamine synthetase purified from sheep brain, clone GS-6 [Millipore] IHC: Donkey anti-goat 488 (Molecular Probes) 1:200

WB: Western blot experiments

IHC: Immunohistochemistry