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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Cell Microbiol. 2010 Oct;12(10):1517–1533. doi: 10.1111/j.1462-5822.2010.01487.x

Fig. 3.

Fig. 3

Effects of SM acyl chain length on the rate of VacA internalization.

AZ-521 cells that had been enriched with either C2-SM or C18-SM were evaluated for internalized Alexa Fluor 488-labeled VacA (50 nM) using flow cytometry, as described under “Experimental Procedures.” The results shown were derived by normalizing the amount of internalized VacA to that measured in control cells that had been mock-pretreated with complete cell medium prior to incubation with VacA (data not shown). Statistical significance was calculated for differences in internalized VacA at each time point between those cells enriched in C2-SM (black bars) and those cells enriched in C18-SM (empty bars).