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. Author manuscript; available in PMC: 2010 Nov 14.
Published in final edited form as: Nat Genet. 2009 Dec 20;42(1):72–76. doi: 10.1038/ng.505

Figure 5. Expression of INF2 variants.

Figure 5

Localization of INF2 variants in undifferentiated cultured human podocytes. Cells were cultured on coverslips coated with type I collagen. INF2 is visualized using a Cy3 conjugated antibody against its C-terminus 7. FITC phalloidin was used to stain F-actin, and nuclei are stained with DAPI. First column: cells expressing wild-type and three different mutant forms of INF2. Second column: INF2 channel. Third column: Phalloidin. Rows: results for WT and three mutant forms of INF2: E184K, S186P, and R218Q. E184K and R218Q mutants show diffuse localization of INF2 and F-actin compared with the perinuclear INF2 staining seen with the WT expressing cells. The S186P looked similar to WT expressing cells, but INF2 expression was more vermiform in appearance. In all three mutants, stress fibers and cortical actin appeared to be less prominent.