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. Author manuscript; available in PMC: 2010 Nov 14.
Published in final edited form as: Biochem Cell Biol. 2010 Feb;88(1):77–88. doi: 10.1139/o09-159

Figure 5. Comparison of the Rvb structures.

Figure 5

To compare the available Rvb structures, high-resolution models such as human Rvb1 and yeast Rvb1/Rvb2 complex were first limited to a resolution of 25Å by applying a Gaussian low-pass filter. A two-dimensional projection along the 6-fold symmetry axis was calculated from the limited resolution 3D structures. In the case of the double-ring EM structure of the human and yeast Rvb1/Rvb2 complexes independent projections were calculated for the top (“Top”) and bottom (“Bottom”) rings (as defined in Fig 2 and 4). The structures are represented at scale thus; their relative size is comparable. In the case of the yeast Rvb1/Rvb2 complex from Gribun et al. (Gribun et al. 2008) the images represent 2D averages of the Rvb1/Rvb2 complex in ATP (left panel) and ATPγS (right panel) obtained upon averaging several hundred 2D projections of these complexes.