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. Author manuscript; available in PMC: 2010 Nov 14.
Published in final edited form as: J Mol Biol. 2010 Aug 14;402(4):638–644. doi: 10.1016/j.jmb.2010.08.015

Figure 4.

Figure 4

Inhibition of dsRNA activation of PKR by VAI and ΔTS. PKR autophosphorylation assays were performed as previously described10;15 in AU 200 + 5 mM MgCl2. 100 nM PKR and variable concentrations of VAI were pre-incubated for 10 minutes at 30°C, followed by addition of 10 ug/ml poly(rI:rC). After 20 minutes, 0.4 mM ATP containing 4µCi γ-32P[ATP] was added and the reaction was incubated for another 40 minutes before quenching with sample loading buffer. PKR autophosphorylation was quantified by phosphorimager analysis and was referenced to samples containing no inhibitor. Each point represents the average of three measurements and the error bars indicate the estimated standard error.