Fig. 3. Lung viral titers and virus-specific antibody-secreting cell responses.
Six out of eleven vaccinated mice (Mock, IM, MN) were sacrificed for analysis of lung viral titers and antibody secreting cell responses at day 4 post challenge as described (Quan et al., 2007). (a) Lung viral titers were determined by a plaque assay with lung homogenate obtained from 6 individual mice. (b) Recall antibody secreting cell responses of spleen cells. Antibody levels were presented as concentrations in culture supernatants (expressed in ng/ml). Values are means ± S.E.M. of individual mice (n=6) after in vitro culture. Asterisk indicates significance between MN and IM groups (*; p<0.05, **; p<0.01, Student's 2-tailed t-test).