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. Author manuscript; available in PMC: 2011 Oct 29.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Sep 26;401(4):527–532. doi: 10.1016/j.bbrc.2010.09.087

Figure 4. Interaction between Abba-IMD alone and Rac1 was not regulated by PDGF.

Figure 4

Cells transfected with GFP-Abba-IMD were grown in 10% serum-containing medium (a and b), 0.1% serum-containing medium (c and d), and 0.1% serum medium plus PDGF for 10 min (e and f). The morphology of the transfected cells was examined by immunofluorescent microscopy after staining with GFP antibody (a, c and e) and phalloidin (b, d and f). Bar: 10 μm. (B) Serum-starved 3T3 cells transiently transfected with GFP-Abba and GFP-Abba-IMD were stimulated with PDGF for 30 min. Rac activation was measured by the pull-down assay. Aliquots of lysates of the samples were also analyzed for Rac input. (C) 3T3 cells stably expressing Myc-RacG12V were transiently transfected with GFP-Abba-IMD and treated with PDGF for 30 min. The interaction between GFP-Abba-IMD and Myc-RacG12V in the treated cells was analyzed as described in the legend of Fig. 4.