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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: Scand J Immunol. 2003 Jul;58(1):33–42. doi: 10.1046/j.1365-3083.2003.01277.x

Figure 1.

Figure 1

Kinetic analysis of T-cell receptor (TCR) donor clones. (A) To determine the steady-state lysis and the lytic kinetics of the cytotoxic T-cell lymphocyte (CTL) clones from which TCR chains were cloned, 5000 51Cr-labelled peptide-pulsed T2 cells (targets, T) were incubated with 10,000 CTLs (effectors, E) for the time periods indicated (E : T of 2 : 1). Identically prepared wells were harvested every 30 min in triplicate and averaged, and corresponding spontaneous and total release wells used to calculate the percent lysis. (B) A CTL assay was carried out as in (A), using the same number of E and a range of T. Supernatants were harvested at 90 min and a Hanes plot of T/Δv versus T was used to calculate Vmax and Km values. Δv is the product of the decimal percent lysis and target cell number divided by the time of the assay in hours (see Materials and methods).