Table 1.

Kinetic values of latency membrane protein-2 peptide cytotoxic T-cell lymphocyte (CTL) clones

Experiment*	Clone	Stimulation†	Number of cycles of re-stim‡	Vmax	Km
1	PL5.04	Standard	1	3527	6524
		Rapid	1	5525	14,756
		Standard	2	4322	9365
		Standard	4	5326	17,000
	PL5.05	Standard	2	2411	4717
2	PL5.05	Rapid-A	1	2053	8706
		Standard-B	1	2222	5733
		Standard-A	2	6060	15,152
		Standard-B	2	6098	13,659
3	PL5.04	Standard	1	7402	17,298
		Standard	3	5000	23,536

^*Each experiment represents a continuous time period of in vitro culture of the CTL clones indicated and the kinetic values calculated during that time period (number of cycles) are reported.

^†Cells were stimulated using either standard or rapid expansion protocols (see Materials and methods}; the suffix '-A' or '-B' denotes clones cultured contemporaneously but with different stimulation methods in the first week of culture.

^‡Each cycle of restimulation lasted from 7 to 10 days from the time the PL5.04 or PL5.O5 clone was brought out of cryopreservation.