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. 2010 Aug 10;19(21):4160–4175. doi: 10.1093/hmg/ddq335

Figure 4.

Figure 4.

Formation of cytoplasmic GFP-FUS aggregates was reversible after heat shock. GFP-FUS(WT, H517Q, R521G, R495X or G515X) was induced for 3 days in HEK-293 cells, and a time series of GFP intensities (acquired as in Fig. 3) was detected in live cells over the indicated time course (hh:mm) upon shifting from 37–42.5°C. Cytoplasmic FUS inclusions assembled most rapidly (by 7–12 min) in cells expressing GFP-FUS(R495X and G5151X) and to a lesser extent in cells expressing GFP-FUS(R521G and H517Q). Aggregate formation was reversible within minutes of a temperature shift back to 37°C, as shown for GFP-FUS(R521G, R495X and G5151X). Scale bar, 10 µm. The full time course appears in Supplementary Material, Videos Fig4video6.avi (G515X), Fig4video7.avi (R495X), Fig4video8.avi (R521G), Fig4video9.avi (H517Q) and Fig4video10.avi (WT).