Figure 5.

Cytoplasmic GFP-FUS in stress granules did not co-localize with adjacent P-bodies. HEK-293 cell lines stably expressing GFP-FUS proteins were treated as described in Figure 2, except that 1 mM sodium arsenite was administered for 2 h and cells were probed with anti-Hedls/GE-1, a marker of P-bodies (red). Scale bar, 10 µm. None of the GFP-FUS proteins co-localized with the P-body marker. However, P-bodies localized in close proximity to stress granules containing the GFP-FUS(R521G, R495X and G515X) variants, and in some cases P-bodies appeared docked to these stress granules (see inset for G515X; scale bar, 1 µm).