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. 2010 Apr 22;47(4):230–235. doi: 10.1136/jmg.2009.069997

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© 2010, Published by the BMJ Publishing Group Limited For permission to use, (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

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Transcriptional activity of WT and mutated TBX20 (I121M) and of the previously published TBX20 mutations I152M and Q195X. (A) Fold activation of a luciferase reporter construct carrying the proximal promoter elements of the Nppa gene after cotransfection of COS7 cells with expression constructs for WT and mutant TBX20 (short isoform). (B) Activation of the Nppa promoter in the presence of mutant and WT TBX20a (full length isoform), NKX2-5 and GATA4. (C, D) Fold activation of a luciferase reporter carrying proximal promoter elements of the Gja5 gene.