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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: J Am Chem Soc. 2008 Dec 24;130(51):17446–17452. doi: 10.1021/ja8055744

Table 1.

Michaelis-Menten parameters of the starting cellulase genes CelN, CelA, CelV and the isolated variants using p-nitrophenyl cellobioside.

Cellulases Km
(mM; mean ± s.d)		 kcat
(s−1; mean ± s.d.)		 kcat/KM
(M−1 s−1; mean ± s.d)
Cel V 4.6 ± 0.3 1.10 × 103 ± 50 2.4 × 105 ± 1.8 × 104
Cel A 7.5 ± 0.7 1.45 × 103 ± 65 1.9 × 105 ± 2 × 104
Cel N 3.4 ± 0.2 1.27 × 103 ± 42 3.7 × 105 ± 2 × 104
Cel_3.7 3.4 ± 0.1 4.5 × 103 ± 132 1.35 × 106 ± 7 × 104
Cel_5.7 2.51 ± 0.08 5.3 × 103 ± 124 2.09 × 106 ± 2 ×104

The standard deviations were calculated from at least three independent measurements.