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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Nat Genet. 2010 Oct 17;42(11):996–999. doi: 10.1038/ng.688

Figure 2.

Figure 2

Haplotype and functional analysis of associated variants in TRAF3IP2. (a) Exon/intron structure of TRAF3IP2 with location of associated SNPs. The four most common haplotypes in a combined set of 2,077 PsA cases and 2,648 control individuals of European origin and their frequency within cases are given. Risk alleles are in bold type. For each haplotype, odds ratios and 95% confidence intervals (in brackets) compare occurrence of this vs. all three other haplotypes. (b) Interaction of wild type TRAF3IP2 (Act1) and PsA-associated variants D10N and R74W with TRAF6 in a mammalian two hybrid dual-luciferase reporter assay. Depicted are means of relative normalized luminescence units reflecting the specific induction of firefly-luciferase by the interaction of the cotransfected ligand pairs TRAF3IP2 and TRAF6 [blue bars]. The investigated TRAF3IP2-variants include constructs harboring either single missense variant (D10N or R74W) or both (D10N+R74W). To exclude autoactivation activity, each prey and bait construct was separately cotransfected with reporter plasmids into HEK293 cells in the absence of any potential interaction partner [open bars]. P53 andTRAF2 as a pair of non-interacting proteins served as an additional independent negative control. Error bars represent 95%-confidence intervals.