Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2011 Nov 5.

Published in final edited form as: Biochem Biophys Res Commun. 2010 Oct 12;402(1):116–122. doi: 10.1016/j.bbrc.2010.09.127

Fig. 3 — E47 and C34 cells were treated with 50–150 mM ethanol for 5 days. MTT assay was carried out to determine cell viability. P<0.05, E47 cells compared to C34 cells (A). CYP2E1 activity was analyzed by determining the oxidation of para-nitrophenol, and results are presented as pmoles/min/mg microsomal protein P< 0.05, E47 cells compared to C34 cells (B). Lipid peroxidation was evaluated by determining the production of malondialdehyde (nmoles/mg cellular protein) (C) by the TBARS assay. Results are from three experiments. P<0.05, E47 cells compared to C34 cells. E47 and C34 cells were treated with ethanol for 5 days and ROS was detected with a fluorescence Total ROS Detection Kit (D). Fifteen different fields in each plate were observed under the fluorescence microscope to determine the fluorescence intensity.

Fig. 3 — E47 and C34 cells were treated with 50–150 mM ethanol for 5 days. MTT assay was carried out to determine cell viability. P<0.05, E47 cells compared to C34 cells (A). CYP2E1 activity was analyzed by determining the oxidation of para-nitrophenol, and results are presented as pmoles/min/mg microsomal protein P< 0.05, E47 cells compared to C34 cells (B). Lipid peroxidation was evaluated by determining the production of malondialdehyde (nmoles/mg cellular protein) (C) by the TBARS assay. Results are from three experiments. P<0.05, E47 cells compared to C34 cells. E47 and C34 cells were treated with ethanol for 5 days and ROS was detected with a fluorescence Total ROS Detection Kit (D). Fifteen different fields in each plate were observed under the fluorescence microscope to determine the fluorescence intensity.