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. Author manuscript; available in PMC: 2011 Nov 5.

Published in final edited form as: Biochem Biophys Res Commun. 2010 Oct 12;402(1):116–122. doi: 10.1016/j.bbrc.2010.09.127

Fig. 4 — E47 and C34 cells were treated with 100 mM ethanol or ethanol plus 3MA (10 mM) for 5 days. Oil Red O Staining was carried out to evaluate the accumulation of lipid droplets (A). The inhibition of autophagy by 3MA was determined with an immunofluorescence method which detects LC3 in the cellular cytosol. The arrows indicate the positive stained autophagy in the cells (B). The contents of TG after ethanol or ethanol plus 3MA treatment (C). * Significantly different P<0.05 compared with C34 cells (n=6).

Fig. 4 — E47 and C34 cells were treated with 100 mM ethanol or ethanol plus 3MA (10 mM) for 5 days. Oil Red O Staining was carried out to evaluate the accumulation of lipid droplets (A). The inhibition of autophagy by 3MA was determined with an immunofluorescence method which detects LC3 in the cellular cytosol. The arrows indicate the positive stained autophagy in the cells (B). The contents of TG after ethanol or ethanol plus 3MA treatment (C). * Significantly different P<0.05 compared with C34 cells (n=6).