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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Mol Microbiol. 2010 Aug 16;77(6):1595–1605. doi: 10.1111/j.1365-2958.2010.07310.x

Figure 1.

Figure 1

Mutation of pst operon leads to activation of PhoB in V. cholerae. V. cholerae strains were grown in MOPS minimal medium supplemented with 6.5 µM or 65 nM KH2PO4 for 10 hours at 37°C. Mean and standard deviation are shown for each time point. All samples were analyzed in triplicate. B) Quantitative RT-PCR (qRT-PCR) analysis of phoA expression. V. cholerae strains were grown in LB at 37°C and RNA collected at OD600=0.3. Expression was normalized to rpoB expression and shown relative to wild-type. The mean and standard deviation for three independent replicates are shown.