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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Mol Microbiol. 2010 Aug 16;77(6):1595–1605. doi: 10.1111/j.1365-2958.2010.07310.x

Figure 2.

Figure 2

PhoB regulates virulence gene expression in V. cholerae. V. cholerae strains were grown in M9 + NRES at 30°C. A) Western blot analysis for CT-B subunit. Secreted proteins were isolated after overnight incubation. Western blot was performed as outlined in Experimental Procedures. B) qRT-PCR analysis of tcpA expression. RNA was collected at OD600=0.3. Expression was normalized to rpoB expression and shown relative to wild-type. The mean and standard deviation for three independent replicates are shown.