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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: Eur J Immunol. 2008 Feb;38(2):587–598. doi: 10.1002/eji.200737817

Figure 2.

Figure 2

A/WySnJ mice are B lymphopenic, whereas A/J and congenic AW.Baffr+/+ mice are not. (A) Schematic representation of Baffr cDNA. Gray shading represents the retrotransposon insertion. (B) AW.Baffr+/+ chromosome 15. Gray shading shows the congenic interval derived from the CAST/Ei strain. (C) Splenocytes were stained with fluorescent antibodies to IgM and IgD, and analyzed for the T1 (IgMhighIgDlow), MZ/T1 (IgMhighIgDint), T2 (IgMhighIgDhigh), and mature B cell subsets (IgMlowIgDhigh). (D) Splenocytes were stained with fluorescent antibodies to IgM, C1qRp, and CD23. The IgM-gated cells were analyzed for the mature B cell subset (CD23highC1qRp−). The C1qRp-gated cells were analyzed for T1 (IgMhighCD23−), T2 (IgMhighCD23+), and T3 (IgMlowCD23+) B cell subsets. The data are representative of six mice per strain.