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CBU1206 functionally complements a yeast erg4 mutant. Cultures induced for 6 h with galactose were spotted in 10-fold dilutions onto untreated plates or plates containing 100 μg of brefeldin A/ml or 0.05 μg of cycloheximide/ml. S. cerevisiae erg4 cells with or without the empty vector pYES-DEST52 were sensitive to both brefeldin A and cycloheximide. In contrast, ERG4 and CBU1206, but not DHCR24, rescued growth.
