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. 2010 Sep 24;192(23):6154–6159. doi: 10.1128/JB.00818-10

FIG. 5.

FIG. 5.

CBU1206 generates ergosterol in yeast. HPLC trace of total sterols extracted from complemented yeast. The dominant sterol in wild-type yeast was ergosterol, while the erg4 mutant accumulated ergosta-5,7,22,24(28)-tetraen-3β-ol. Expression of ERG4 and CBU1206, but not DHCR24, in erg4 yeast resulted in the generation of ergosterol. A commercially available standard was used to determine the retention time of ergosterol.