TABLE 1.
Primer | Sequence (5′-3′) |
---|---|
YES-DEST52recFforGFP | TGAGTTTAAACCCGCTGATCCTAGAGGGC |
YES-DEST52recRforGFP | ACCTTCGAAGGGCCCTCTAGATCGAACC |
YES-GFP-recF | GGGCCCTTCGAAGGTATGGCTAGCAAAGGAGAAGAAC |
YES-GFP-recR | GCGGGTTTAAACTCATTATTTGTAGAGCTCATCCATGC |
yeastpENTR1206for | CACCAATAATGTCTCGCGTTACCCCACTTGATCAAAAATT |
CBU1206rev | AAAAATAAACGGAATAAATAACCAAGG |
yeastpENTRerg4for | CACCAATAATGGCAAAGGATAATAGTGAGAAGCTG |
Erg4rev | GAAAACATAAGGAATAAAGACGTAAGGG |
yeastpENTRDHCR24for | CACCAATAATGTCTGAGCCCGCCGTGTCGCTGGCC |
DHCR24rev | GTGCCTGGCGGCCTTGCAGATC |