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. 2010 Oct 1;192(23):6230–6239. doi: 10.1128/JB.00655-10

FIG. 1.

FIG. 1.

Characterization of the fliL mutant strain. (A) Swimming of FS3 (ΔfliL3::aadA) and FS3 carrying the wild-type fliL allele cloned in pRK451. WS8 was included as a positive control. (B) Electron micrograph of FS3 cells showing the presence of flagella. (C) Western blot analysis of the supernatant fraction obtained after strong vortexing of WS8, FS3, and FS3/pRK-fliL cultures. A polyclonal anti-FliC antibody was used for detection. Strain SP7 (ΔrpoN2::Kan) was included as a negative control.