TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| S17-1 | recA endA thi hsdR RP4-2-Tc::Mu::Tn7; Tpr Smr | 50 |
| LMG194 | Protein expression strain | Invitrogen |
| TOP10 | Cloning strain | Invitrogen |
| Rosetta | Protein expression strain, Cmr | Novagen |
| R. sphaeroides | ||
| WS8 | Wild-type strain, spontaneous Nalr | 62 |
| SP7 | WS8 derivative ΔrpoN2::Kan | 49 |
| SP13 | WS8 derivative ΔfleQ1::Kan | 48 |
| SP18 | WS8 derivative flgC1::Kan | 47 |
| FS3 | WS8 derivative ΔfliL3::aadA | This study |
| FS4 | WS8 derivative ΔmotB1::Kan | This study |
| FS5 | WS8 derivative ΔfliL3::aadA ΔmotB1::Kan | This study |
| SUP1 | WS8 derivative ΔfliL3::aadA motB (A67E) | This study |
| SUP2 | WS8 derivative ΔfliL3::aadA motB (A67E) | This study |
| SUP3 | WS8 derivative ΔfliL3::aadA motB (A56E) | This study |
| SUP4 | WS8 derivative ΔfliL3::aadA motB (F63L) | This study |
| SUP5 | WS8 derivative ΔfliL3::aadA motB (A67D) | This study |
| SUP6 | WS8 derivative ΔfliL3::aadA motB (A67T) | This study |
| SUP7 | WS8 derivative ΔfliL3::aadA motB (F63L) | This study |
| SUP8 | WS8 derivative ΔfliL3::aadA motB (A67G) | This study |
| S. cerevisiae AH109 | Yeast reporter strain, for HIS3, ADE2, and lacZ | Clontech |
| Plasmids | ||
| pTZ19R | Cloning vector, Apr, pUC derivative | Pharmacia |
| pRK415 | pRK404 derivative | 33 |
| pJQ200mp18 | Mobilizable suicide vector for R. sphaeroides | 51 |
| pBAD/HisB | Expression vector of six-His-tagged proteins | Invitrogen |
| pBAD/HisC | Expression vector of six-His-tagged proteins | Invitrogen |
| pBAD-fliL | pBAD/HisB expressing His6-FliLp | This study |
| pGEX-4T-2 | Expression vector for GST gene fusion | Amersham |
| pEGFP | Expression vector for GFP gene fusion | Clontech |
| pUC4K | Source of the Kanr cassette | Pharmacia |
| pPIRL | Vector that encodes tRNAs for rare codons | 6 |
| pRK-fliL | pRK415 derivative, fliL gene | Laboratory collection |
| pRK-motB | pRK415 derivative, expressing motB+ | This study |
| pRK-motBsup1 | pRK415 derivative, expressing motBsup1 | This study |
| pRK-motBsup2 | pRK415 derivative, expressing motBsup2 | This study |
| pRK-motBsup3 | pRK415 derivative, expressing motBsup3 | This study |
| pRK-motBsup4 | pRK415 derivative, expressing motBsup4 | This study |
| pRK-motBsup5 | pRK415 derivative, expressing motBsup5 | This study |
| pRK-motBsup6 | pRK415 derivative, expressing motBsup6 | This study |
| pRK-motBsup7 | pRK415 derivative, expressing motBsup7 | This study |
| pRK-motBsup8 | pRK415 derivative, expressing motBsup8 | This study |
| pRK-fliLΔ15 | pRK415 derivative, expressing fliLΔ15 gene | This study |
| pRK_FliL-GFP | pRK415 derivative, expressing fliL-gfp | This study |
| pRK_GFP-FliL | pRK415 derivative, expressing gfp-fliL | This study |
| pGBKT7 | GAL4 DNA binding domain, TRP1 | Clontech |
| pGADT7 | GAL4 activation domain, LEU2 | Clontech |
| pGBD-fliLp | pGBKT7 derivative expressing GAL4 BD-FliLp | This study |
| pGAD-fliLp | pGADT7 derivative expressing GAL4 AD-FliLp | This study |
| pGBD-motBp | pGBKT7 derivative expressing GAL4 BD-MotBp | This study |