TABLE 1.
Properties of the P. aeruginosa strains used in this study
| Straina | Patient age (yr) when isolate was collected | Growth rate (doubling time in min) | MIC (μg/ml) of: |
||
|---|---|---|---|---|---|
| Ofloxacinb | Carbenicillin | Tobramycin | |||
| Parent (AMT0023-30) | 0.5 | 72.7 | 1 (1) | 64 | 1.0 |
| AMT0023-31 | 7.7 | 81.0 | 8 | 128 | 32.0 |
| AMT0023-31 | 7.7 | 74.3 | 8 | 128 | 32.0 |
| AMT0023-35 | 8.0 | 80.3 | 8 | 128 | 32.0 |
| 96-mo isolate (AMT0023-34) | 8.0 | 70.8 | 8 | 128 | 32.0 |
| ΔmexXY mutant (KLE2000) | 8.0 | 62.2 | 1 (2) | 128 | 2.0 |
| AMT0047-2 | 0.8 | 100.0 | 2 (1) | 1,600 | 0.8 |
| AMT0047-3 | 7.3 | 122.1 | 0.5 (1) | 400 | 0.4 |
| AMT0060-3 | 7.7 | 62.2 | 8 (14) | 800 | 4.0 |
| AMT0060-2 | 15.4 | 71.2 | 16 (16) | 100 | 1.0 |
| AMT0066-3 | 7.2 | 82.0 | 2 (0.25) | 200 | 3.125 |
| AMT0066-1 | 15.2 | 86.8 | 4 (4) | 800 | >50.0 |
| AMT0071-2 | 3.0 | 54.3 | 2 (2) | 400 | 0.8 |
| AMT0071-3 | 9.1 | 43.3 | 1 (2) | 400 | 0.8 |
| AMT0074-1 | 9.2 | 64.5 | 16 (16) | 100 | 3.125 |
| AMT0074-3 | 19.6 | 86.8 | 16 (16) | 200 | 1.56 |
| AMT0076-3 | 10.8 | 58.9 | 2 (1) | 1,600 | 6.25 |
| AMT0076-1 | 19.6 | 56.4 | 1 (1) | 800 | 0.4 |
| AMT0101-3 | 1.0 | 39.8 | 2 (2) | 400 | 1.56 |
| AMT0101-1 | 9.6 | 112.9 | 0.25 (0.25) | 200 | 1.56 |
| AMT0033-2 | 1.1 | 43.7 | 4 (2) | 800 | 1.56 |
| AMT0033-3 | 13.2 | 36.1 | 2 (2) | 800 | >50.0 |
| AMT0041-1 | 5.6 | 56.4 | 4 (4) | 800 | 3.125 |
| AMT0041-3 | 12.8 | 46.7 | 16 (16) | >1,600 | >50.0 |
| AMT0075-1 | 7.1 | 58.2 | 1 (1) | 400 | 0.8 |
| AMT0075-4 | 23.4 | 61.9 | 2 (2) | 400 | 0.8 |
a
Strain pairs from individual patients are grouped by spacing. Strain pairs in boldface indicate that a hip mutant emerged during the course of infection with no increase in antimicrobial resistance.
b
For ofloxacin, MICs for persister progeny are shown in parentheses.