TABLE 2.

Mutational analysis of DivIB^a

Strain	Mutation	Source or reference	Growth at 30°C	Growth at 48°C
RSA9	Wild type	31	+	+
RSA24	E122A	This study	+	+
RSA16	L139A	This study	+	+
RSA17	E140A	This study	+	+
RSA18	N141A	This study	+	+
RSA25	S186A	This study	+	+
RSA26	E187A	This study	+	+
RSA27	P192A	This study	+	+
RSA19	Y203A	This study	+	+
RSA21	N205A	This study	+	+
RSA22	D206A	This study	+	+
RSA30	Y224A	31	+	+
RSA31	P225A	31	+	+
RSA28	Y246A	This study	+	+/−b

^aResults of a complementation assay (31) that monitors the ability of mutant divIB expressed from an ectopic locus to complement a divIB null strain at permissive (30°C) and nonpermissive (48°C) growth temperatures. All strains showed growth (+).

^bLysis at 24 h.