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(A) Genetic map of pRAS3 plasmids with the differences in the plasmid backbones of pRAS3.1 and pRAS3.2 shown below the map. (B) Nucleotide sequence of the four 22-bp repeats of pRAS3.1 indicating the location of the unique BstEII site used to construct plasmids with different numbers of iterons. (C) Agarose gel showing oriV regions of pRAS3 containing different numbers of iterons. (D) Diagram showing derivatives of pRAS3.1 with different numbers of iterons.
