FIG. 1.

Various constructs of MzrA proteins and their abilities to complement a ΔmzrA mutation. The transmembrane (TM) or the signal sequence (SS) regions are shown as rounded rectangles. Shaded rectangles show the NarX TM domain and the PhoA signal sequence. The last three recombinant proteins contained an extra serine (S) residue that was inserted due to the creation of a restriction site in the corresponding DNA. Complementation was tested by examining the ability of the expressed proteins to inhibit (+) or not inhibit (−) OmpF and MalE synthesis, as shown in Fig. 2 and 3. WT, wild type.