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Effects of N- and C-terminal deletions of MzrA on OmpF, MalE, and MzrAFLAG. (A and B) Western blot analysis of whole-cell protein extracts isolated from freshly grown cultures containing plasmids, as shown in the figure. Expression of various recombinant proteins from pBAD24 was induced by the addition of 0.2% l-arabinose for 3 h. Membrane blots were probed with OmpF/OmpC/OmpA (A) or TolC/MalE and FLAG (B) antibodies.
