FIG. 1.

Oritavancin but not its precursor chloroeremomycin or vancomycin disrupts bacterial membrane integrity, leading to concomitant killing of hVISA ATCC 700698. (A to D) Effects of oritavancin and comparator agents on membrane depolarization (A), membrane permeability (B), cell viability (C), and the correlation between membrane depolarization and cell killing (D). Drug concentrations: control (✳); oritavancin at 4 μg/ml (▪), 8 μg/ml (○) and 16 μg/ml (•); 16 μg/ml chloroeremomycin (⋄); 16 μg/ml vancomycin (⧫). Note that the chloroeremomycin and vancomycin curves overlap in panel A and with the control curve in panel C. The limit of detection (200 CFU) is indicated as a dashed line in panel C.