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(A) B. anthracis spores were treated with different concentrations of 6-TG, and germination was monitored by decreases in the optical density at 580 nm. The IC50 for 6-TG (1 mM) was determined based on these data. (B) Macrophages were treated with B. anthracis spores and different concentrations of 6-TG. Macrophage killing was monitored based on increases of PI fluorescence intensity.
