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. 2010 Oct 4;54(12):5329–5336. doi: 10.1128/AAC.01029-10

FIG. 5.

FIG. 5.

Macrophage protection by 6-TG and d-Ala combinations. Macrophages were treated with B. anthracis spores, and killing was monitored at 5 h postinfection based on increased PI fluorescence intensity. Addition of 1 mM d-Ala or 0.1 mM 6-TG significantly reduced macrophage killing. In contrast, treatment with 50 μM d-Ala or 0.1 μM 6-TG did not prevent cell killing. Simultaneous treatment with 50 μM d-Ala and 0.1 μM 6-TG protected macrophages from B. anthracis cytotoxicity. Error bars represent standard deviations of six independent measurements. *, significantly decreased killing in the presence of both 50 μM d-Ala and 0.1 μM 6-TG compared to samples treated with 50 μM d-Ala alone (P < 0.005) or samples treated with 0.1 μM 6-TG alone (P < 0.0001).